Human PVRIG Protein, Ultra Low Endotoxin
Catalog No: PVRIG-HP003
- Species
- Human
- Expression System
- HEK293
- Tag
- an mFc (IgG2a)
- Activity
- Activity verified
Product overview
Recombinant Human PVRIG Protein is expressed in HEK293 cells with an mFc (IgG2a) tag at the C-terminus. It contains amino acid residues Thr41-Asp171 (UniProt accession: Q6DKI7).
Product Details
- Molecular Aliases
- C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1
- Protein Length
- Thr41-Asp171
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.03 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human PVRIG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PVRIG is greater than 90% as determined by SEC-HPLC.
Immobilized Human Nectin-2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA (QC Test).
Immobilized Human PVRIG, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
Human PVRIG, mFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-2, His Tag with an affinity constant of 87.49 nM as determined in SPR assay (Biacore T200).
Background
Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.
References
- PVRIG and PVRL2 Are Induced in Cancer and Inhibit CD8 T-cell Function.
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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