Human PSMP Protein
Catalog No: PSMP-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Human PSMP Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Lys37-Ser139 (UniProt accession: Q1L6U9).
Product Details
- Molecular Aliases
- Msmp; Psmp; PC3-secreted microprotein
- Protein Length
- Lys37-Ser139
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human PSMP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PSMP is greater than 95% as determined by SEC-HPLC.
Background
PC3-secreted microprotein (PSMP)/microseminoprotein (MSMP) is a novel chemotactic cytokine and its receptor is CCR2. PSMP was highly expressed in fibrotic/cirrhotic tissues from patients with different etiologies of liver disease and in the 3 experimental mouse models of fibrosis. Damage-associated molecular pattern molecules HMGB-1 and IL-33 induced hepatocytes to produce PSMP. PSMP promotes liver fibrosis through inflammatory macrophage infiltration, polarization and production of proinflammatory cytokines, as well as direct activation of hepatic stellate cells via its receptor CCR2.
References
- She S, Wu X, Zheng D, Pei X, Ma J, Sun Y, Zhou J, Nong L, Guo C, Lv P, Song Q, Zheng C, Liang W, Huang S, Li Q, Liu Z, Song Z, Li Y, Zhang Y, Kong W, You H, Xi J, Wang Y. PSMP/MSMP promotes hepatic fibrosis through CCR2 and represents a novel therapeutic target. J Hepatol. 2020 Mar;72(3):506-518. doi: 10.1016/j.jhep.2019.09.033. Epub 2019 Dec 6. PMID: 31813573.
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