Human NKp46/NCR1/CD335 Protein
Catalog No: CD335-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human NKp46/NCR1/CD335 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln22-Asn255 (UniProt accession: O76036-1).
Product Details
- Molecular Aliases
- CD335; Ly94; NCR1; NKp46; MAR-1; NKP46FLJ99094;MAR1
- Protein Length
- Gln22-Asn255
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 53.2 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human NKp46 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human NKp46 is greater than 95% as determined by SEC-HPLC.
Immobilized Human NKp46, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Nkp46 Antibody, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA.
Background
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR).These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells.
References
- Glasner A, Isaacson B, Viukov S, et al. Increased NK cell immunity in a transgenic mouse model of NKp46 overexpression.[J]. Sci Rep, 2017, 7(1):13090. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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