Human NKp30/NCR3/CD337 Protein
Catalog No: CD337-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human NKp30/NCR3/CD337 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu19-Thr138 (accession: AAH52582).
Product Details
- Molecular Aliases
- CD337; LY117; NCR3; NKp30; 1C7; MALS
- Protein Length
- Leu19-Thr138
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human NKp30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human NKp30 is greater than 95% as determined by SEC-HPLC.
Immobilized Human NKp30, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-NKp30 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA (QC Test).
Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H6, His Tag (Cat. BH7-HM176) with an affinity constant of 48.67 nM as determined in SPR assay (Biacore T200).
Loaded Human NKp30, hFc Tag on Anti-His-Biosensor can bind Human B7-H6, His Tag (Cat. BH7-HM176) with an affinity constant of 0.56 µM as determined in BLI assay.
Background
NKp30, along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors". These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation.
References
- Strandmann E P V, Shatnyeva O, Hansen H P. NKp30 and its ligands: Emerging players in tumor immune evasion from natural killer cells[J]. Annals of Translational Medicine,
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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