Human Nectin-3/CD113 Protein
Catalog No: CD113-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human Nectin-3/CD113 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gly58-Asp400 (UniProt accession: Q9NQS3).
Product Details
- Molecular Aliases
- CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3
- Protein Length
- Gly58-Asp400
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 63.63 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Nectin-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Nectin-3 is greater than 95% as determined by SEC-HPLC.
Immobilized Human Nectin-3, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-1, His Tag (Cat. NEC-HM401) with the EC50 of 77.8ng/ml determined by ELISA. (QC Test)
Background
The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids.
References
- Inagaki, M. Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development[J]. Development, 132(7):1525-1537.
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