Human Mucin-1/MUC-1 Protein
Catalog No: MUC1-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human Mucin-1/MUC-1 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ala961-Ser1152 (UniProt accession: P15941-1).
Product Details
- Molecular Aliases
- MUC1; MUC-1; Mucin-1; Mucin1; CA 15-3; Episialin; H23AG; KL-6; PEMT; PUM; PEM; EMA; CD227;;CA15-3; KL6
- Protein Length
- Ala961-Ser1152
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 46.61 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Mucin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Mucin-1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human Mucin-1, hFc Tag at 0.5μg/ml(100μl/well) on the plate. Dose response curve for Biotinylated Anti-Mucin-1 Antibody, hFc Avi tag with the EC50 of 23.1ng/ml determined by ELISA.
Background
Mucin 1 (MUC1) is a transmembrane protein mainly expressed by epithelial and hematopoietic cells. It has a receptor-like structure, which can sense the external environment and activate intracellular signal transduction pathways through its cytoplasmic domain.
References
- Ballester B, Milara J, Cortijo J. The role of mucin 1 in respiratory diseases. Eur Respir Rev. 2021 Feb 2;30(159):200149. doi: 10.1183/16000617.0149-2020. PMID: 33536260; PMCID: PMC9488590. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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