Human M-CSF/CSF-1 Protein
Catalog No: MCSF-HP001
- Species
- Human
- Expression System
- HEK293
- Activity
- Activity verified
Product overview
Recombinant Human M-CSF/CSF-1 Protein is expressed in HEK293 cells without tag. It contains amino acid residues Glu33-Asn190 (UniProt accession: P09603-1).
Product Details
- Molecular Aliases
- CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim
- Protein Length
- Glu33-Asn190
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 18.40 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.05 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human M-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.
Immobilized Human M-CSF, No Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 13.5ng/ml determined by ELISA. (QC Test)
Measured in a cell proliferation assay using RAW264.7 cells. The ED50 for this effect is 1-10 ng/mL.
Human M-CSF, No Tag immobilized on CM5 Chip can bind Human M-CSF R, hFc Tag with an affinity constant of 2.06 nM as determined in SPR assay (Biacore T200).
Background
CSF-1, also known as M-CSF, is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
References
- Cannarile M A, Weisser M, Jacob W, et al. Colony-stimulating factor 1 receptor (CSF1R) inhibitors in cancer therapy[J]. Journal for ImmunoTherapy of Cancer, 2017, 5(1):53.
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