Human LTBR Protein
Catalog No: LTBR-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human LTBR Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln31-Met227 (UniProt accession: P36941-1).
Product Details
- Molecular Aliases
- TNFCR; TNFR3; TNFRSF3; LTBR; CD18; TNFR2-RP; TNFR-RP; LT-BETA-R; TNF-R-III; D12S370; LT-β-R;TNFR2RP;LT-b-R
- Protein Length
- Gln31-Met227
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human LTBR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human LIGHT, His Tag at 1 μg/ml (100 μl/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 13.8 ng/ml determined by ELISA (QC Test).
Immobilized Human LTBR, hFc Tag at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-LTBR Antibody, hFc Tag with the EC50 20.7 ng/ml determined by ELISA.
Background
Components of lymphotoxin beta receptor (LTBR)-associated signaling complexes, including TRAF2, TRAF3, NIK, IKK1, and IKK2 have been shown to participate in the coupling of LTBR to NFkappaB. TRAF3 functions as a negative regulator of LTBR signaling via both canonical and non-canonical NFkappaB pathways by two distinct mechanisms. These effects of TRAF3 depletion did not require LTBR signaling and were consistent with autonomous activation of the non-canonical NFkappaB pathway.
References
- Bista P, Zeng W, Ryan S, Bailly V, Browning JL, Lukashev ME. TRAF3 controls activation of the canonical and alternative NFkappaB by the lymphotoxin beta receptor. J Biol Chem. 2010 Apr 23;285(17):12971-8. doi: 10.1074/jbc.M109.076091. Epub 2010 Feb
- PMID: 20185819; PMCID: PMC2857099.
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