Human LILRB1/CD85j/ILT2 Domain1&2 Protein
Catalog No: LILRB1-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human LILRB1/CD85j/IL-T2 Domain1&2 Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gly24-Gly221 (UniProt accession: Q8NHL6-1).
Product Details
- Molecular Aliases
- ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4
- Protein Length
- Gly24-Gly221
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human LILRB1 Domain1&2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human LILRB1 Domain1&2 is greater than 95% as determined by SEC-HPLC.
Immobilized Human LILRB1 Domain1&2, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-LILRB1 Antibody were added into Biotinylated Human HLA-G Complex Tetramer, His Tag: Human LILRB1 Domain1&2, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 34.6ng/ml.
Human HLA-G Tetramer captured on CM5 Chip can bind Human LILRB1 Domain1&2, His Tag with an affinity constant of 1.9μM as determined in SPR assay (Biacore T200).
Background
LILRB1, also known as CD85j and IL-T2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens.
References
- Apps R, Gardner L, Sharkey A, et al. A homodimeric complex of HLA-G on normal trophoblast cells modulates antigen-presenting cells via LILRB1 [J]. European Journal of Immunology, 2007, 37(7):1924-1937. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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