Human LAIR1/CD305 Protein
Catalog No: CD305-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human LAIR1/CD305 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln22-His163 (UniProt accession: Q6GTX8-1).
Product Details
- Molecular Aliases
- CD305; HLAIR1; LAIR1; LAIR-1
- Protein Length
- Gln22-His163
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 42.4 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human LAIR1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human LAIR1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human LAIR1, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-LAIR1 Antibody, hFc Tag with the EC50 of 42.6ng/ml determined by ELISA (QC Test).
Immobilized Human Collagen I, No Tag at 2μg/ml (50μl/well) on the plate. Dose response curve for Human LAIR1, hFc Tag with the EC50 of 1.17μg/ml determined by ELISA.
Background
LAIR1 (leukocyte-associated Ig-like receptor-1, designated CD305) is an approximately 40 kDa type I transmembrane inhibitory glycoprotein belonging to the Ig superfamily.LAIR1 functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation.
References
- Clinical significance of LAIR1 (CD305) as assessed by flow cytometry in a prospective series of patients with chronic lymphocytic leukemia[J]. Haematologica, 2014, 99(5):881-887. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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