Human ITPRIPL1/CD3L1 Protein
Catalog No: ITPRIPL1-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Human ITPRIPL1/CD3L1 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues His25-Gly103 (UniProt accession: Q6GPH6-1).
Product Details
- Molecular Aliases
- KIAA1754L; ITPRIPL1; CD3L1
- Protein Length
- His25-Gly103
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.24 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human ITPRIPL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ITPRIPL1 is greater than 95% as determined by SEC-HPLC.
Background
Inositol 1,4,5-trisphosphate receptor-interacting protein-like 1 (ITPRIPL1), a single-pass type I membrane protein with uncharacterized functions, exhibits normal testis-enriched expression and widespread overexpression in various human cancers. ITPRIPL1 possesses a distinct ECD and is particularly prevalent in tumors exhibiting low or no PD-L1 expression. ITPRIPL1 functions as an inhibitory ligand of CD3ε, and its expression inhibits T cells in the tumor microenvironment.
References
- Deng S, Shi J, Sun Y, Quan Y, Shen Z, Wang Y, Li H, Xu J. Development of a monoclonal antibody to ITPRIPL1 for immunohistochemical diagnosis of non-small cell lung cancers: accuracy and correlation with CD8+ T cell infiltration. Front Cell Dev Biol. 2023 Dec 19;11:1297211. doi: 10.3389/fcell.2023.1297211. PMID: 38188019; PMCID: PMC10770237. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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