Human IL-5 R alpha/CD125 Protein
Catalog No: CD125-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-5 R alpha/CD125 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Asp21-Trp342 (UniProt accession: Q01344-1).
Product Details
- Molecular Aliases
- CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560; IL5R alpha; IL5R subunit alpha
- Protein Length
- Asp21-Trp342
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 62.73 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-5 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-5 R alpha is greater than 90% as determined by SEC-HPLC.
Immobilized Human IL-5, His Tag (Cat. IL5-HM101) at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. (QC Test)
Loaded Human IL-5 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-5, His Tag with an affinity constant of 14.80 nM as determined in BLI assay.
Background
Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology. This is the receptor for interleukin-5. The alpha chain binds to IL5.
References
- Garcia G, Taille C, Laveneziana P, et al. Anti-interleukin-5 therapy in severe asthma[J]. European Respiratory Review, 2013, 22(129):251-257.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.