Human IL-4 R alpha/CD124 Protein
Catalog No: CD124-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-4 R alpha/CD124 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Met26-His232 (UniProt accession: P24394-1).
Product Details
- Molecular Aliases
- CD124; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124;IL4R alpha; IL4R subunit alpha; IL4Ra
- Protein Length
- Met26-His232
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.7 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-4 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-4 R alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-4, No Tag (Cat. IL4-HM001) at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA. (QC Test)
Immobilized Human IL-4 R alpha, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-IL-4R alpha Antibody, hFc Avi tag with the EC50 of 28.8ng/ml determined by ELISA.
Loaded Human IL-4 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-4, No Tag (Cat. IL4-HM001) with an affinity constant of 0.54 nM as determined in BLI assay.
Background
Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells.
References
- Ghoreschi K, Thomas P, Breit S, et al. Interleukin-4 therapy of psoriasis induces Th2 responses and improves human autoimmune disease[J]. Nature Medicine, 2002, 9(1):40-46. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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