Human IL-4 Protein
Catalog No: IL4-HP001
- Species
- Human
- Expression System
- HEK293
- Activity
- Activity verified
Product overview
Recombinant Human IL-4 Protein is expressed in HEK293 cells without tag. It contains amino acid residues His25-Ser153 (UniProt accession: P05112-1).
Product Details
- Molecular Aliases
- IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1
- Protein Length
- His25-Ser153
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 14.96 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-4 is greater than 95% as determined by SEC-HPLC.
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.01-0.35 ng/mL (QC Test)
Immobilized Human IL-4, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) with the EC50 of 4.4ng/ml determined by ELISA. (QC Test)
Loaded Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) on ProA-Biosensor can bind Human IL-4, No Tag with an affinity constant of 0.54 nM as determined in BLI assay.
Background
Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.
References
- Ghoreschi K, Thomas P, Breit S, et al. Interleukin-4 therapy of psoriasis induces Th2 responses and improves human autoimmune disease[J]. Nature Medicine, 2002, 9(1):40-46.
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