Human IL-27RA/TCCR Protein
Catalog No: TCCR-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-27RA/TCCR Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gly34-Lys516 (UniProt accession: Q6UWB1).
Product Details
- Molecular Aliases
- IL-27RA; TCCR; IL27RA; IL-27R-alpha; IL27R alpha; WSX-1; WSX1; ZcytoR1; CRL1; IL-27 RA; IL27 RA; IL27R-alpha
- Protein Length
- Gly34-Lys516
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-27RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.1ng/ml determined by ELISA (QC Test).
Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).
Background
A multicenter, web-based Thyroid Cancer and Tumor Collaborative Registry (TCCR, allows for the collection and management of various data on thyroid cancer (TC) and thyroid nodule (TN) patients. The TCCR is coupled with OpenSpecimen, an open-source biobank management system, to annotate biospecimens obtained from the TCCR subjects.
References
- Shats O, Goldner W, Feng J, Sherman A, Smith RB, Sherman S. Thyroid Cancer and Tumor Collaborative Registry (TCCR). Cancer Inform. 2016 May 3;15:73-9. doi: 10.4137/CIN. S32470. PMID: 27168721; PMCID: PMC4856228.
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