Human IL-20RA Protein
Catalog No: IL20RA-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-20RA Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Val30-Lys250 (UniProt accession: Q9UHF4-1).
Product Details
- Molecular Aliases
- IL-20R-alpha; IL-20RA; CRF2-8; IL-20R1; ZcytoR7; FLJ40993; IL20R-alpha; IL20RA; IL20R1
- Protein Length
- Val30-Lys250
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-20RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-20RA is greater than 90% as determined by SEC-HPLC.
Human IL-20RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-20, His Tag with an affinity constant of 1.05 uM as determined in SPR assay (Biacore T200). (QC Test)
Background
IL-20RA was highly expressed in the tumor tissue of CRC and related to the advanced stage. IL-20RA was involved in regulating oncogenic and immune pathways and affecting the expression of genes related to cell proliferation and immune evasion in CRC. Super-enhancer inhibition by JQ-1 or iBET-151 suppressed the growth of tumor cells and inhibited the expression of IL-20RA.
References
- Yu D, Yang X, Lin J, Cao Z, Lu C, Yang Z, Zheng M, Pan R, Cai W. Super-Enhancer Induced IL-20RA Promotes Proliferation/Metastasis and Immune Evasion in Colorectal Cancer. Front Oncol. 2021 Jul 15;11:724655. doi: 10.3389/fonc.2021.724655. PMID: 34336707; PMCID: PMC8319729.
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