Human IL-12 R beta 1/CD212 Protein
Catalog No: CD212-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-12 R beta 1/CD212 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Cys24-Glu540 (UniProt accession: P42701-1).
Product Details
- Molecular Aliases
- IL-12R-beta-1; IL-12RB1; IL12R; IL12RB; CD212; IL-12R subunit beta-1; IL-12R-BETA1; MGC34454;IL-12R-β-1; IL-12R subunit β-1; IL-12R-β1;IL12R-beta-1; IL12RB1; IL12R subunit beta-1; IL12R-BETA1; IL12R-β-1; IL12R subunit β-1; IL12R-β1;IL-12R-b-1; IL12R-b1; IL-12R subunit b-1; IL12R subunit b1; IL-12R-b1; IL-12R-beta 1; IL12R-beta 1
- Protein Length
- Cys24-Glu540
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 83.85 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-12 R beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human IL-12B, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA.
Background
Human IL12RB1 is an autosomal gene that is essential for mycobacterial disease resistance and T cell differentiation. Lung and T cell IL12RB1 expression is allele-biased, and the extent to which cells express one IL12RB1 allele is unaffected by activation. Furthermore following its expression the IL12RB1 pre-mRNA is processed into either IL12RB1 Isoform 1 (IL12Rβ1, a positive regulator of IL12 responsiveness) or IL12RB1 Isoform 2 (a protein of heretofore unknown function).
References
- Reeme AE, Claeys TA, Aggarwal P, Turner AJ, Routes JM, Broeckel U, Robinson RT. Human IL12RB1 expression is allele-biased and produces a novel IL12 response regulator. Genes Immun. 2019 Mar;20(3):181-197. doi: 10.1038/s41435-018-0023-2. Epub 2018 Mar
- PMID: 29599514; PMCID: PMC6165718.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.