Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein
Catalog No: HLAE0103-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide (accession: P13747(HLA-E*01:03) & P61769(B2M) &VMAPRTLVL).
Product Details
- Molecular Aliases
- HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1
- Protein Length
- Gly22-Thr302
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
Immobilized Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test).
Human NKG2A&CD94, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag with an affinity constant of 17.61 nM as determined in SPR assay (Biacore T200).
Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag with an affinity constant of 1.65 μM as determined in SPR assay (Biacore T200).
The purity of Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer is greater than 90% as determined by SEC-HPLC.
Background
HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
References
- (1) Kanevskiy L, Erokhina S, Kobyzeva P, Streltsova M, Sapozhnikov A, Kovalenko E. Dimorphism of HLA-E and its Disease Association. Int J Mol Sci. 2019 Nov 4;20(21):5496. doi: 10.3390/ijms20215496. PMID: 31690066; PMCID: PMC6862560.
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