Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein
Catalog No: WT1-HP001
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and RMFPNAPYL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &RMFPNAPYL).
Product Details
- Molecular Aliases
- MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer is greater than 95% as determined by SEC-HPLC.
Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 43.4ng/ml determined by ELISA. (QC Test)
Loaded Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc-Avi tag on ProA-Biosensor can bind Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag with an affinity constant of 53.70 nM as determined in BLI assay.
Background
The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium. The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.
References
- (1)Dao T, Pankov D, Scott A, et al. Therapeutic bispecific T-cell engager antibody targeting the intracellular oncoprotein WT1[J]. Nature Biotechnology, 2015.
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