Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer Protein
Catalog No: TDT-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYDKTKRI peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &ALYDKTKRI).
Product Details
- Molecular Aliases
- DNA nucleotidylexotransferase; terminal deoxynucleotidyl transferase
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer is greater than 95% as determined by SEC-HPLC.
Immobilized Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 11.3ng/ml determined by ELISA.
Background
The terminal deoxynucleotidyl transferase (TdT) belongs to the X family of DNA polymerases. This unusual polymerase catalyzes the template-independent addition of random nucleotides on 3'-overhangs during V(D)J recombination. The biological function and intrinsic biochemical properties of the TdT have spurred the development of numerous oligonucleotide-based tools and methods, especially if combined with modified nucleoside triphosphates.
References
- Sarac I, Hollenstein M. Terminal Deoxynucleotidyl Transferase in the Synthesis and Modification of Nucleic Acids. Chembiochem. 2019 Apr 1;20(7):860-871. doi: 10.1002/cbic.201800658. Epub 2019 Jan 25. PMID: 30451377.
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