Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer Protein
Catalog No: MART1-HP001
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and EAAGIGILTV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &EAAGIGILTV).
Product Details
- Molecular Aliases
- MART1; LB39-AA; SK29-AA; Melan-A; MLANA
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.
Immobilized Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 10.1ng/ml determined by ELISA.
Background
Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, also recognized by cytotoxicity T lymphocytes as a marker. Mart-1 is considered to play a critical role in the immunotherapy for melanoma.
References
- Wang J, Jia R, Yao Y, Cun B, Huang X, Gu P, Ge S, Fan X. Differential expression of Mart-1 in human uveal melanoma cells. Mol Med Rep. 2011 Sep-Oct;4(5):799-803. doi: 10.3892/mmr.2011.504. Epub 2011 Jun 7. PMID: 21667026.
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