Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein
Catalog No: MAGEA1-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &KVLEYVIKV).
Product Details
- Molecular Aliases
- MAGE-A1; MAGE A1; CT1.1; MAGE-1 antigen; MAGE-1; MZ2-E; MAGE1; MAGE1A
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 53-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer is greater than 95% as determined by SEC-HPLC.
Background
MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors.
References
- Wang D, Wang J, Ding N, Li Y, Yang Y, Fang X, Zhao H. MAGE-A1 promotes melanoma proliferation and migration through C-JUN activation. Biochem Biophys Res Commun. 2016 May 13;473(4):959-965. doi: 10.1016/j.bbrc.2016.03.161. Epub 2016 Apr 1. PMID: 27045082.
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