Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Tetramer Protein
Catalog No: HPV16E6-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KLPQLCTEL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &KLPQLCTEL).
Product Details
- Molecular Aliases
- Human papillomavirus type 16; HPV16; E6;
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
Background
Human papillomavirus (HPV) 16 infection is a necessary condition for the pathogenesis and development of cervical cancer. The E6 protein is expressed by the HPV16 E6 gene and promotes malignant phenotype transformation, which is an important mechanism for the occurrence and development of cervical cancer.
References
- Zhang L, Lai Y, Sun Y, Xu B, Qiang X, Zhou X, Wang T. HPV16 E6 regulates the proliferation, invasion, and apoptosis of cervical cancer cells by downregulating miR-504. Transl Cancer Res. 2020 Dec;9(12):7588-7595. doi: 10.21037/tcr-20-2913. PMID: 35117358; PMCID: PMC8799100.
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