Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer Protein
Catalog No: HBV-HP003
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Human HBV(HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FLLTRILTI peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) & FLLTRILTI peptide).
Product Details
- Molecular Aliases
- Hepatitis B virus; MHC; HBV
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer is greater than 90% as determined by SEC-HPLC.
Background
Hepatitis B virus (HBV), is the leading cause of liver diseases infecting an estimated 240 million persons worldwide. The HBV prevalence rates are variables between different countries, with an high level of endemicity in the south-eastern part of Europe. Seven main HBV-D subgenotypes have been described until now (D1-D7).
References
- Ciccozzi M, Ciccaglione AR, Lo Presti A, Equestre M, Cella E, Ebranati E, Gabanelli E, Villano U, Bruni R, Yalcinkaya T, Tanzi E, Zehender G. Evolutionary dynamics of HBV-D1 genotype epidemic in Turkey. J Med Virol. 2014 Jan;86(1):109-16. doi: 10.1002/jmv.23740. Epub 2013 Oct 24. PMID: 24243521.
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