PROVETOP

Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer Protein

Catalog No: HBV-HP001

Species
Human
Expression System
E. coli
Tag
His, Avi

Product overview

Recombinant Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and FLLTRILTI peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &FLLTRILTI).

Product Details

Molecular Aliases
Hepatitis B virus; MHC; HBV
Protein Length
Gly25-Thr305
Expression System
E. coli
Theoretical Molecular Weight
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin
Less than 1 EU per μg by the LAL method.
Buffer / Formulation
Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
State
Liquid
Storage Conditions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data Display

Bis-Tris PAGE
HBV-HP001 Bis-Tris-PAGE-white result

Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC
HBV-HP001 SEC-HPLC result

The purity of Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer is greater than 95% as determined by SEC-HPLC.

Background

Hepatitis B virus (HBV), is the leading cause of liver diseases infecting an estimated 240 million persons worldwide. The HBV prevalence rates are variables between different countries, with an high level of endemicity in the south-eastern part of Europe. Seven main HBV-D subgenotypes have been described until now (D1-D7).

References

  1. Ciccozzi M, Ciccaglione AR, Lo Presti A, Equestre M, Cella E, Ebranati E, Gabanelli E, Villano U, Bruni R, Yalcinkaya T, Tanzi E, Zehender G. Evolutionary dynamics of HBV-D1 genotype epidemic in Turkey. J Med Virol. 2014 Jan;86(1):109-16. doi: 10.1002/jmv.23740. Epub 2013 Oct 24. PMID: 24243521.

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