Human Her3/ErbB3 Protein
Catalog No: HER3-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human Her3/ErbB3 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ser20-Thr643 (UniProt accession: P21860).
Product Details
- Molecular Aliases
- ErbB3; ErbB-3; HER3; HER3c-erbB-3; LCCS2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; ERBB3;HER3c-erbB3; MDA-BF1; p180ErbB3; p45sErbB3; p85sErbB3
- Protein Length
- Ser20-Thr643
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 95.4 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Her3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human Her3 is greater than 95% as determined by SEC-HPLC.
Immobilized Human Her3, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.33μg/ml determined by ELISA (QC Test).
Immobilized Human Her3, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Her3 Antibody, hFc Tag with the EC50 of 10.7ng/ml determined by ELISA.
Loaded Human NRG1 beta 1, His Tag (Cat. NRG-HM1B1) on Anti-His-Biosensor can bind Human Her3, hFc Tag with an affinity constant of 2.61 nM as determined in BLI assay.
Background
Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).
References
- Yonesaka K, Haratani K, Hirotani K, et al. Abstract 44: U3-1402, a novel HER3-targeting ADC, and a novel DNA topoisomerase I inhibitor inhibit the growth of non-small cell lung cancer with EGFR mutation[J]. Cancer Research, 2017, 77(13 Supplement):44-44.
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