Human GP1BB Protein
Catalog No: GP1BB-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Human GP1BB Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Pro27-Cys147 (UniProt accession: P13224-1).
Product Details
- Molecular Aliases
- GP-Ib beta; GPIb-beta; GPIbB; Gp1bb; BDPLT1; BS; CD42c; GP1BB; GPIbb; GPIbbeta;GP-Ib β; GPIb-β; GPIbβ;GP-Ib b; GPIb-b; GPIb beta
- Protein Length
- Pro27-Cys147
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 47-52 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human GP1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human GP1BB is greater than 95% as determined by SEC-HPLC.
Background
The glycoprotein (GP) Ib-IX complex is the receptor on platelet surfaces that mediates their adhesion to subendothelium. It comprises three polypeptides (GP Ib alpha, GP Ib beta, GP IX), each of which belongs to a superfamily of proteins containing conserved leucine-rich motifs. Association between GP Ib alpha and GP Ib beta was demonstrated biochemically on immunoblots of detergent lysates of CHO alpha beta cells; electrophoresis under nonreducing conditions revealed the two subunits to be covalently linked through a disulfide bond.
References
- López JA, Weisman S, Sanan DA, Sih T, Chambers M, Li CQ. Glycoprotein (GP) Ib beta is the critical subunit linking GP Ib alpha and GP IX in the GP Ib-IX complex. Analysis of partial complexes. J Biol Chem. 1994 Sep 23;269(38):23716-21. PMID:
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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