Human FGFR2 alpha (IIIb) Protein
Catalog No: FGFR2-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human FGFR2 alpha (IIIb) Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Arg22-Glu378 (UniProt accession: P21802-3).
Product Details
- Molecular Aliases
- FGF R2a; FGFR2 alpha; KGFR; CD332; BBDS; BEK; BFR-1; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25; FLJ98662
- Protein Length
- Arg22-Glu378
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 66.4 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human FGFR2 alpha (IIIb) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC.
Immobilized Human FGF-7, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human FGFR2 alpha (IIIb), hFc Tag with the EC50 of 0.87μg/ml determined by ELISA. (QC Test)
Immobilized Human FGFR2 alpha (IIIb), hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA.
Background
Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform.
References
- Katoh, Masaru. FGFR inhibitors: Effects on cancer cells, tumor microenvironment and whole-body homeostasis (Review)[J]. International Journal of Molecular Medicine, 2016.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.