Human Fc epsilon RI alpha/FCER1a Protein
Catalog No: FCER1A-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Val26-Gln205 (UniProt accession: P12319-1).
Product Details
- Molecular Aliases
- Fc-epsilon RI-alpha; FcERI; FCER1A; FCE1A
- Protein Length
- Val26-Gln205
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Fc epsilon RI alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Fc epsilon RI alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Human Fc epsilon RI alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IgE, His-Avi tag with the EC50 of 23.6ng/ml determined by ELISA. (QC Test)
Immobilized Human Fc epsilon RI alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
Background
Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.
References
- Sanak M, Potaczek DP, Nizankowska-Mogilnicka E, Szczeklik A. Genetic variability of the high-affinity IgE receptor alpha subunit (Fc epsilon RI alpha) is related to total serum IgE levels in allergic subjects. Allergol Int. 2007 Dec;56(4):397-401. doi: 10.2332/allergolint.R-07-145. Epub 2007 Nov
- PMID: 17965580.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.