Human DNAM-1/CD226 Protein
Catalog No: DNAM1-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human DNAM-1/CD226 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Glu19-Asn247 (UniProt accession: Q15762).
Product Details
- Molecular Aliases
- CD226 molecule; CD226; DNAM1; PTA1; TLiSA1
- Protein Length
- Glu19-Asn247
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 52.8 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human DNAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human DNAM-1 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human CD155, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).
Human DNAM-1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 μM as determined in SPR assay (Biacore T200).
Loaded Human DNAM-1, hFc Tag on Anti-Human lgG Fc Gen II (HFc II) -Biosensor can bind Biotinylated Human CD155, His-Avi tag (Cat. CD1-HM455B) with an affinity constant of 0.75 nM as determined in BLI assay.
Background
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
References
- Yu X, Harden K, C Gonzalez L, et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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