Human/Cynomolgus Activin RIIA/ACVR2A Protein
Catalog No: RIIA-HF001
- Species
- Human/Cyno
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human/Cynomolgus Activin RIIA/ACVR2A Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ala20-Pro135 (accession: P27037-1(Human) / G7PKJ4(Cynomolgus)).
Product Details
- Molecular Aliases
- ACTR-IIA; ACTRIIA; ACVR2A; ACVR2
- Protein Length
- Ala20-Pro135
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of W 39.35 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human/Cynomolgus Activin RIIA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human/Cynomolgus Activin RIIA is greater than 95% as determined by SEC-HPLC.
Immobilized Human/Mouse/Rat Activin A, No Tag (Cat. ACV-HM001) at 1μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA (QC Test).
Immobilized Human/Mouse/Rat GDF-8, No Tag (Cat. GDF-HM008) at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA.
Immobilized Human Activin B, No Tag (Cat. ACV-HM00B) at 2μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
Immobilized Human Activin E, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 0.95μg/ml determined by ELISA.
Human/Cynomolgus Activin RIIA, hFc Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag (Cat. GDF-HM008) with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
Loaded Human/Cynomolgus Activin RIIA, hFc Tag on ProA-Biosensor can bind Human Activin B, No Tag (Cat. ACV-HM00B) with an affinity constant of 0.25 nM as determined in BLI assay.
Background
Activin A receptor type 2A (ACVR2A) is a membrane receptor in the transforming growth factor- beta (TGF-β signaling pathway, which is involved in the regulation of cell proliferation, migration, and apoptosis. Loss of ACVR2A has an important role in cancer progression and distant metastasis and may serve as a prognostic marker in patients with colon cancer.
References
- Zhuo C, Hu D, Li J, Yu H, Lin X, Chen Y, Zhuang Y, Li Q, Zheng X, Yang C. Downregulation of Activin A Receptor Type 2A Is Associated with Metastatic Potential and Poor Prognosis of Colon Cancer. J Cancer. 2018 Sep 8;9(19):3626-3633. doi: 10.7150/jca.26790. PMID: 30310521; PMCID: PMC6171025.
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