Human CLEC7A Protein
Catalog No: CLEC7A-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human CLEC7A Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Thr66-Met201 (UniProt accession: Q9BXN2-2).
Product Details
- Molecular Aliases
- Beta-glucan receptor; DC-associated C-type lectin 1; Dectin-1; Dectin1; CD369; BGR; CLECSF12; DECTIN1; CANDF4
- Protein Length
- Thr66-Met201
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 41.79 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CLEC7A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CLEC7A is greater than 95% as determined by SEC-HPLC.
Immobilized Human CLEC7A, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CLEC7A Antibody, hFc Avi tag with the EC50 of 5.0ng/ml determined by ELISA. (QC Test)
Background
Dendritic cell-associated C-type lectin-1 (Dectin-1), also known as β-glucan receptor is an emerging pattern recognition receptor (PRR) which belongs to the family of C-type lectin receptor (CLR). Dectin-1 signaling axis has been suggested to be targeted as an effective therapeutic strategy for cancers. Dectin-1 has also been elucidated ascetically in the heart, respiratory, intestinal, neurological and developmental disorders.
References
- Kalia N, Singh J, Kaur M. The role of dectin-1 in health and disease. Immunobiology. 2021 Mar;226(2):152071. doi: 10.1016/j.imbio.2021.152071. Epub 2021 Feb 5. PMID: 33588306.
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