Human CEACAM-3/CD66d Protein
Catalog No: CD66D-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human CEACAM-3/CD66d Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Lys35-Gly155 (UniProt accession: P40198-1).
Product Details
- Molecular Aliases
- CEACAM3; CD66d; CGM1; CEA;
- Protein Length
- Lys35-Gly155
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 39.85 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CEACAM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CEACAM-3 is greater than 95% as determined by SEC-HPLC.
Immobilized Human CEACAM-7, His Tag (Cat. CEA-HM107) at 2μg/ml (100μl/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 28.7ng/ml determined by ELISA. (QC Test)
Background
The human granulocyte-specific receptor carcinoembryonic antigen-related cell adhesion molecule (CEACAM)3 is critically involved in the opsonin-independent recognition of several bacterial pathogens. CEACAM3-mediated phagocytosis depends on the integrity of an ITAM-like sequence within the cytoplasmic domain of CEACAM3 and is characterized by rapid stimulation of the GTPase Rac.
References
- Schmitter T, Pils S, Sakk V, Frank R, Fischer KD, Hauck CR. The granulocyte receptor carcinoembryonic antigen-related cell adhesion molecule 3 (CEACAM3) directly associates with Vav to promote phagocytosis of human pathogens. J Immunol. 2007 Mar 15;178(6):3797-805. doi: 10.4049/jimmunol.178.6.3797. PMID: 17339478.
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