Human CD83 Protein
Catalog No: CD83-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human CD83 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Thr20-Ala143 (UniProt accession: Q01151-1).
Product Details
- Molecular Aliases
- CD83; HB15; BL11; CD83 molecule
- Protein Length
- Thr20-Ala143
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD83 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD83 is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD83, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD83 Antibody, hFc Tag with the EC50 25.3ng/ml determined by ELISA (QC Test).
Background
CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.
References
- Li Z, Ju X, Silveira PA, Abadir E, Hsu WH, Hart DNJ, Clark GJ. CD83: Activation Marker for Antigen Presenting Cells and Its Therapeutic Potential. Front Immunol. 2019 Jun 7;10:1312. doi: 10.3389/fimmu.2019.01312. PMID: 31231400; PMCID: PMC6568190. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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