Human CD48/SLAMF2 Protein
Catalog No: CD48-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human CD48/SLAMF2 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln27-Ser220 (UniProt accession: P09326-1).
Product Details
- Molecular Aliases
- CD48 molecule; CD48; SLAMF2; BCM1; BLAST; BLAST1; BLAST1TCT.1;- mCD48; MEM-102; TCT.1;MEM102
- Protein Length
- Gln27-Ser220
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 48.9 kDa. Due to glycosylation, the protein migrates to 62-72 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD48 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD48 is greater than 95% as determined by SEC-HPLC.
Immobilized Human 2B4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34μg/ml determined by ELISA. (QC Test)
Loaded Human CD48, hFc Tag on ProA-Biosensor can bind Human 2B4, His Tag with an affinity constant of 0.51 µM as determined in BLI assay.
Background
CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules.CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors.
References
- Velikovsky C A, Deng L, Chlewicki L K, et al. Structure of Natural Killer Receptor 2B4 Bound to CD48 Reveals Basis for Heterophilic Recognition in Signaling Lymphocyte Activation Molecule Family[J]. Immunity, 2007, 27(4):0-584. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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