Human CD47 Protein
Catalog No: CD47-HP004
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human CD47 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln19-Pro139 (UniProt accession: Q08722-1).
Product Details
- Molecular Aliases
- CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
- Protein Length
- Gln19-Pro139
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD47 is greater than 95% as determined by SEC-HPLC.
Immobilized Human SIRP alpha, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
Human CD47, hFc Tag captured on CM5 Chip via Protein A can bind Human Sirpa V2, His Tag with an affinity constant of 13.8nM as determined in SPR assay (Biacore T200).
Background
CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
References
- Weiskopf K, Ring A M, Ho C C M, et al. Engineered SIRP? Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies[J]. Science, 2013, 341(6141):88-91. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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