Human CD3E/CD3 epsilon Protein
Catalog No: CD3E-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human CD3E/CD3 epsilon Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Asp23-Asp126 (UniProt accession: P07766).
Product Details
- Molecular Aliases
- CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
- Protein Length
- Asp23-Asp126
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 12.6 kDa. Due to glycosylation, the protein migrates to 14-18 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Immobilized Human CD3E, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 9.0 ng/ml determined by ELISA. (QC Test)
Loaded Anti-CD3 Antibody, hFc Tag on ProA-Biosensor can bind Human CD3E, His Tag with an affinity constant of 2.14 nM as determined in BLI assay.
Background
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
References
- Kroesen B J, Nieken J, Sleijfer D T, et al. Approaches to lung cancer treatment using the CD3E×GP-2-directed Bispecific Monoclonal Antibody BIS-1[J]. Cancer Immunology Immunotherapy, 1997, 45(3-4):203-206..
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