Human CD300LF Protein
Catalog No: CD300LF-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Human CD300LF Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Thr20-Ser156 (UniProt accession: Q8TDQ1-1).
Product Details
- Molecular Aliases
- CLM-1; IREM-1; IgSF13; CD300F; IGSF13; NKIR; CD300f; CD300LF; CLIM1; DIgR2; LMIR3; NKIR; Pigr3;CLM1; IREM1
- Protein Length
- Thr20-Ser156
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 42.1 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD300LF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD300LF is greater than 95% as determined by SEC-HPLC.
Background
Murine norovirus (MNoV) is an important model of human norovirus (HNoV) and mucosal virus infection more broadly. CD300lf as a proteinaceous receptor for MNoV. Interestingly, its paralogue CD300ld was also sufficient for MNoV infection in vitro. CD300lf is essential for both oral and parenteral MNoV infection and to elicit anti-MNoV humoral responses in vivo. human CD300lf (huCD300lf) is not essential for HNoV infection, nor does huCD300lf inhibit binding of HNoV virus-like particles to glycans.
References
- Graziano VR, Walker FC, Kennedy EA, Wei J, Ettayebi K, Strine MS, Filler RB, Hassan E, Hsieh LL, Kim AS, Kolawole AO, Wobus CE, Lindesmith LC, Baric RS, Estes MK, Orchard RC, Baldridge MT, Wilen CB. CD300lf is the primary physiologic receptor of murine norovirus but not human norovirus. PLoS Pathog. 2020 Apr 6;16(4):e1008242. doi: 10.1371/journal.ppat.1008242. PMID: 32251490; PMCID: PMC7162533. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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