Human CD30 Ligand/TNFSF8 Protein
Catalog No: CD30-HF002
- Species
- Human
- Expression System
- HEK293
- Tag
- an mFc (IgG2a)
- Activity
- Activity verified
Product overview
Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed in HEK293 cells with an mFc (IgG2a) tag at the N-terminus. It contains amino acid residues Gln63-Asp234 (UniProt accession: P32971-1).
Product Details
- Molecular Aliases
- CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG
- Protein Length
- Gln63-Asp234
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD30 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD30, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test).
Background
CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
References
- Van d W C A, Pileri S A, Feldman A L, et al. Understanding CD30 biology and therapeutic targeting: a historical perspective providing insight into future directions[J]. Blood Cancer Journal, 2017, 7(9):e603. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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