Human CD23/Fc epsilon RII Protein
Catalog No: CD23-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
Product overview
Recombinant Human CD23/Fc epsilon RII Protein is expressed in HEK293 cells with a His tag at the N-terminus. It contains amino acid residues Asp48-Ser321 (UniProt accession: P06734-1).
Product Details
- Molecular Aliases
- BLAST-2; CD23; CD23A; CLEC4J; FCE2; IGEBF; FCER2; FcεRII; Fcr; Fcgr; Fc receptor; CLEC-6; CLECSF8; MCL; MGC40078; MPCL; Fcer2a; Ly-42
- Protein Length
- Asp48-Ser321
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 31.8 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD23 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD23 is greater than 95% as determined by SEC-HPLC.
Background
CD23 is the low-affinity receptor for immunoglobulin (Ig)E and plays important roles in the regulation of IgE responses. CD23 can be cleaved from cell surfaces to yield a range of soluble CD23 (sCD23) proteins that have pleiotropic cytokine-like activities.
References
- Liu C, Richard K, Wiggins M, Zhu X, Conrad DH, Song W. CD23 can negatively regulate B-cell receptor signaling. Sci Rep. 2016 May 16;6:25629. doi: 10.1038/srep25629. PMID: 27181049; PMCID: PMC4867583.
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