Human CD229/SLAMF3 Protein
Catalog No: CD229-HF002
- Species
- Human
- Expression System
- HEK293
- Tag
- an mFc (IgG1)
Product overview
Recombinant Human CD229/SLAMF3 Protein is expressed in HEK293 cells with an mFc (IgG1) tag at the C-terminus. It contains amino acid residues Lys364-Asp452 (UniProt accession: Q9HBG7-1).
Product Details
- Molecular Aliases
- SLAMF3; CD229; LY9; AI893573; Lgp100; T100
- Protein Length
- Lys364-Asp452
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.56 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Human CD229 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD229 is greater than 90% as determined by SEC-HPLC.
Background
CD229 was strongly and homogeneously overexpressed on the PC of patients with monoclonal gammopathy of undetermined significance (MGUS), smoldering myeloma, MM, and PC leukemia. CD229 was particularly overexpressed on those PC showing an abnormal phenotype such as expression of CD56. Most importantly, CD229 was also highly expressed on those cells in the patients' BM displaying the phenotype of chemotherapy-resistant and myeloma-propagating cells.
References
- Yousef S, Kovacsovics-Bankowski M, Salama ME, Bhardwaj N, Steinbach M, Langemo A, Kovacsovics T, Marvin J, Binder M, Panse J, Kröger N, Luetkens T, Atanackovic D. CD229 is expressed on the surface of plasma cells carrying an aberrant phenotype and chemotherapy-resistant precursor cells in multiple myeloma. Hum Vaccin Immunother. 2015;11(7):1606-11. doi: 10.1080/21645515.2015.1046658. PMID: 26001047; PMCID: PMC4514151.
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