Human BTLA Protein, Ultra Low Endotoxin
Catalog No: BTLA-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human BTLA Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Lys31-Ser150 (UniProt accession: Q7Z6A9-1).
Product Details
- Molecular Aliases
- CD272; BTLA; BTLA1; FLJ16065; MGC129743
- Protein Length
- Lys31-Ser150
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human BTLA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BTLA is greater than 95% as determined by SEC-HPLC.
Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test).
Immobilized Human BTLA, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-BTLA Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA.
Loaded Human HVEM, His Tag (Cat. HVE-HM111) on Anti-His-Biosensor can bind Human BTLA, hFc Tag with an affinity constant of 3.67 nM as determined in BLI assay.
Loaded Biotinylated Anti-BTLA Antibody, hFc Tag on Streptavidin-Biosensor can bind Human BTLA, hFc Tag with an affinity constant of 0.67 nM as determined in BLI assay.
Background
B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.
References
- Haymaker C L, Wu R C, Ritthipichai K, et al. BTLA marks a less-differentiated tumor-infiltrating lymphocyte subset in melanoma with enhanced survival properties[J]. OncoImmunology, 2015, 4(8):e1014246. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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