Human BST2 Protein
Catalog No: BST2-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human BST2 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Asn49-Ser161 (UniProt accession: Q10589-1).
Product Details
- Molecular Aliases
- BST-2; HM1.24 antigen; CD317; BST2; NPC-A-7; PDCA-1; TETHERIN;NPC-A7; PDCA1
- Protein Length
- Asn49-Ser161
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human BST2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BST2 is greater than 95% as determined by SEC-HPLC.
Immobilized Human BST2, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-BST2 Antibody, hFc Tag with the EC50 of 40.0ng/ml determined by ELISA. determined by ELISA. (QC Test)
Loaded Biotinylated Anti-BST2 Antibody, hFc Tag on Streptavidin-Biosensor can bind Human BST2, hFc Tag with an affinity constant of 4.04 nM as determined in BLI assay.
Background
Interferon-induced BST2 (bone marrow stromal cell antigen 2) inhibits viral replication by tethering enveloped virions to the cell surface to restrict viral release and by inducing the NFKB-dependent antiviral immune response. BST2 expression was significantly increased during porcine epidemic diarrhea virus (PEDV) infection of Vero cells by IRF1 targeting its promoter. Both the BST2 and N protein interacted with the E3 ubiquitin ligase MARCHF8/MARCH8 and the cargo receptor.
References
- Kong N, Shan T, Wang H, Jiao Y, Zuo Y, Li L, Tong W, Yu L, Jiang Y, Zhou Y, Li G, Gao F, Yu H, Zheng H, Tong G. BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy. Autophagy. 2020 Oct;16(10):1737-1752. doi: 10.1080/15548627.2019.1707487. Epub 2019 Dec
- PMID:
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.