Human Beta Klotho Protein
Catalog No: BETAKLOTHO-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human Beta Klotho Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met30-Thr983 (UniProt accession: Q86Z14).
Product Details
- Molecular Aliases
- betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; MGC142213;βKlotho; β-klotho; klotho β like; Klotho β
- Protein Length
- Met30-Thr983
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS, 8% trehalose (pH 7.4).
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Beta Klotho on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human Beta Klotho is greater than 95% as determined by SEC-HPLC.
Immobilized Human Beta Klotho at 2μg/ml (100μl/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA (QC Test).
Immobilized Human Beta Klotho at 2μg/ml (100μl/Well). Dose response curve for Anti-Beta Klotho Antibody2, hFc Tag with the EC50 of 34.6ng/ml determined by ELISA (QC Test).
Immobilized Human FGF21, mFc Tag (Cat. FGF-HM621) at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.75μg/ml determined by ELISA.
Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
Human FGFR4, hFc Tag (Cat. FGF-HM2R4) captured on CM5 Chip via Protein A can Human Beta Klotho, His Tag with an affinity constant of 66.52 nM as determined in SPR assay (Biacore T200).
Loaded Anti-Beta Klotho Antibody1, hFc Tag on ProA-Biosensor can bind Human Beta Klotho, His Tag with an affinity constant of 9.40 nM as determined in BLI assay.
Loaded Anti-Beta Klotho Antibody2, hFc Tag on ProA-Biosensor can bind Human Beta Klotho, His Tag with an affinity constant of 0.40 nM as determined in BLI assay.
Loaded Human Beta Klotho, His Tag on Aint-His-Biosensor can bind Human FGF21, mFc Tag (Cat. FGF-HM621) with an affinity constant of 0.16 nM as determined in BLI assay.
Background
Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.
References
- Bono BS, Koziel Ly NK, Miller PA, Williams-Ikhenoba J, Dumiaty Y, Chee MJ. Spatial distribution of beta-klotho mRNA in the mouse hypothalamus, hippocampal region, subiculum, and amygdala. J Comp Neurol. 2022 Feb 10. doi: 10.1002/cne.25306. Epub ahead of print. PMID: 35143049.
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