Human BAFF/TNFSF13B/CD257 Trimer Protein
Catalog No: CD257-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- monomeric Fc, Flag
- Activity
- Activity verified
Product overview
Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed in HEK293 cells with monomeric Fc tag and Flag tag at the N-terminus. It contains amino acid residues Thr141-Leu285 trimer design (UniProt accession: Q9Y275-1).
Product Details
- Molecular Aliases
- BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1;TALL1delta BAFF; TALL1Delta4BAFF
- Protein Length
- Thr141-Leu285
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 74.9 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human BAFF (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BAFF (Trimer) is greater than 95% as determined by SEC-HPLC.
The purity of Human BAFF Trimer is greater than 95% and the molecular weight of this protein is around 75-95 kDa as determined by SEC-MALS.
Immobilized Human BAFF Trimer, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
Immobilized Human BAFF Trimer, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-BAFF Antibody, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA.
Measured by a reporter gene assay using BAFFR Effector Reporter Cell line. The ED50 for this effect is < 80 ng/mL.
Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF (Trimer), hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
Loaded Human BAFFR, His Tag on Anti-His-Biosensor can bind Human BAFF (Trimer), monomeric Fc-Flag Tag with an affinity constant of 0.98 nM as determined in BLI assay.
Background
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
References
- Bossen C, Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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