Human B7-2/CD86 Protein, Ultra Low Endotoxin
Catalog No: CD86-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human B7-2/CD86 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu26-Pro247 (UniProt accession: P42081-1).
Product Details
- Molecular Aliases
- CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2
- Protein Length
- Leu26-Pro247
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B7-2 is greater than 95% as determined by SEC-HPLC.
Immobilized Human CTLA-4, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human B7-2, hFc Tag with the EC50 of 125.7ng/ml determined by ELISA. (QC Test)
Loaded Human CTLA-4, His Tag on Anti-His-Biosensor can bind Human B7-2, hFc Tag with an affinity constant of 9.35 nM as determined in BLI assay.
Background
B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
References
- Bhatia S, Edidin M, Almo SC, Nathenson SG. B7-1 and B7-2: similar costimulatory ligands with different biochemical, oligomeric and signaling properties. Immunol Lett. 2006 Apr 15;104(1-2):70-5. doi: 10.1016/j.imlet.2005.11.019. Epub 2005 Dec
- PMID:
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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