Human A2AR Nanodisc Protein
Catalog No: A2AR-HP001
- Species
- HEK293
- Expression System
- C-terminal His tag
- Tag
- C-terminal His, contains Met1-Ser412 (UniProt accession: P29274)
Product overview
Recombinant Human A2AR Nanodisc protein is expressed in HEK293 cells with a C-terminal His tag and contains Met1-Ser412 (UniProt accession: P29274). The nanodisc format presents the full-length multi-pass membrane protein in a membrane-like environment for antibody-binding and assay-development research.
Product Details
- Molecular Aliases
- A2aR; Adenosine A2a R; ADORA 2; ADORA2; ADORA2A; RDC8
- Protein Length
- Met1-Ser412
- Expression System
- C-terminal His tag
- Theoretical Molecular Weight
- 45.5 kDa
- Endotoxin
- <1 EU/μg (LAL assay)
- Buffer / Formulation
- 0.22 μm filtered solution in PBS with 200 mM L-arginine, pH 7.4. Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Store at -80°C for up to 6 months. Minimize freeze-thaw cycles.
Data Display
Immobilized Human A2AR Nanodisc, His Tag at 2 μg/mL (100 μL/well) on the plate. The dose-response curve for Anti-A2AR Antibody, mFc Tag gave an EC50 of 0.15 μg/mL by ELISA (QC test).
Human A2AR Nanodisc, His Tag captured on a CM5 chip via anti-His antibody bound Anti-A2AR Antibody, mFc Tag with an affinity constant of 0.32 nM by SPR (Biacore T200).
Anti-A2AR Antibody loaded on a Protein A biosensor bound Human A2AR Nanodisc, His Tag with an affinity constant of 4.44 nM by BLI (Gator).
Background
Adenosine is a neuromodulator in the adult central nervous system. Membrane-bound receptors for adenosine include the A1, A2A, A2B and A3 receptor subtypes. Expression of A2A adenosine receptor mRNA in cranial ganglia, the carotid body and the intermediate lobe of the pituitary gland suggests additional sites of adenosine action during development and in adults.
References
- Weaver DR. A2a adenosine receptor gene expression in developing rat brain. Brain Res Mol Brain Res. 1993 Dec;20(4):313-327. doi: 10.1016/0169-328X(93)90058-W. PMID: 8114618.
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