Human 2B4/CD244/SLAMF4 Protein, Ultra Low Endotoxin
Catalog No: 2B4-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Cys22-Arg221 (UniProt accession: Q9BZW8-2).
Product Details
- Molecular Aliases
- 2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4
- Protein Length
- Cys22-Arg221
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human 2B4 is greater than 90% as determined by SEC-HPLC.
Immobilized Human 2B4, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA.
Loaded Human CD48, hFc Tag on ProA-Biosensor can bind Human 2B4, His Tag with an affinity constant of 0.51 µM as determined in BLI assay.
Background
2B4 (CD244) is expressed by memory-phenotype CD8 T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
References
- Velikovsky C A, Deng L, Chlewicki L K, et al. Structure of Natural Killer Receptor 2B4 Bound to CD48 Reveals Basis for Heterophilic Recognition in Signaling Lymphocyte Activation Molecule Family[J]. Immunity, 2007, 27(4):0-584. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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