Cynomolgus LILRB4/CD85k/ILT3 Protein
Catalog No: LILRB4-CP001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Cynomolgus LILRB4/CD85k/ILT3 Protein Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gln22-Glu259 (accession: XP_015297198.1).
Product Details
- Molecular Aliases
- HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K
- Protein Length
- Gln22-Glu259
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 27.2 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Cynomolgus LILRB4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus LILRB4 is greater than 95% as determined by SEC-HPLC.
Immobilized Cynomolgus LILRB4, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.
Background
LILRB4,also known as CD85k and LIR-5, ILT3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
References
- Samuel J, Heyu C, Mi D, et al. A Novel Anti-LILRB4 CAR-T Cell for the Treatment of Monocytic AML[J]. Molecular Therapy, 2018:S1525001618303721-. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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