Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer Protein
Catalog No: GP100-HP005
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119(mB2M) and YLEPGPVTA peptide (accession: A0A140T933(Human HLA-A*02:01 α1 & α2) & P01897(Mouse H-2Ld α3) & P01887(Mouse B2M) &YLEPGPVTA).
Product Details
- Molecular Aliases
- HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100
- Protein Length
- Gly25-Thr206
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 47.90 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer is greater than 95% as determined by SEC-HPLC.
Immobilized Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test).
Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag with an affinity constant of 67.84 nM as determined in SPR assay (Biacore T200).
Background
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
References
- (1)Abad J D, Wrzensinski C, Overwijk W, et al. T-Cell Receptor Gene Therapy of Established Tumors in a Murine Melanoma Model[J]. Journal of Immunotherapy, 2008, 31(1):1-6.
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